CHARACTERIZATION OF RUBISCO FROM VARIOUS PLANT SOURCES FOR EFFICIENT CATALYTIC ACTIVITY AGAINST CARBON SEQUESTRATION Authors: S. Jenny Angel , C. HEMALATHA AND M. PRIYADHARSHINI
ABSTRACT
To combat the tremendous increase in atmospheric carbon dioxide level a wide number of
technologies are being explored. The selection of natural and less destructive way would be by
using plants as they are the most natural sinks present in Earth that have the ability to sequester
carbon dioxide. This is mainly carried out by RuBisco, a protein found in all phototrophic forms
as a major enzyme involved in photosynthesis. The present work focuses on isolating RuBisco
protein from plant species taking into account one from each C3 and C4 plants. This was done in
order to determine the effective plant with high carbon dioxide sequestering property. The
experimental study was done both in normal and carbon dioxide stressed conditions. Rubisco was
isolated from tomato and sugarcane plant and it was characterized by SDS PAGE, FT-IR and
HPLC technique. To determine the carbon capture by the plant protein CHNSO analysis was
carried out. The plant that absorbed higher concentration of carbon was can be identified.
Keywords: Solanum lycopersicum; Saccharum officinarum; RuBisco, Carbon dioxide; global
warming; C4 plants Publication date: 01/04/2024 https://ijbpas.com/pdf/2024/April/MS_IJBPAS_2024_7938.pdfDownload PDFhttps://doi.org/10.31032/IJBPAS/2024/13.4.7938