ABSTRACT

Present study involves development of a liquid chromatography method for the estimation of Mutual Prodrug of Propyphenazone and Flurbiprofen. RP-HPLC method was developed using Column C18 (Shimadzu Shim-pack Gist (250* 4.6 mm, 5 ?m) as stationary phase and Acetonitrile:Water (90:10) as mobile phase. Flow rate was kept at 1 mL/min and detection was performed using UV detector at 245 nm. The retention Mutual Prodrug was observed to be 4.5 min. The linear regression analysis data for the calibration plot showed a good linear relationship for Mutual Prodrug over a concentration range of 10–50 ?g/mL. The proposed method was validated with respect to linearity, accuracy, precision and robustness. The developed method can be used for routing quality control evaluation of Mutual Prodrug in bulk. Keywords: RP-HPLC, Prodrug, NSAIDs, Propyphenazone, Flurbiprofen, Validation
Publication date: 01/03/2024

https://ijbpas.com/pdf/2024/March/MS_IJBPAS_2024_7860.pdf

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https://doi.org/10.31032/IJBPAS/2024/13.3.7860