ABSTRACT

A Simple and precise UV- spectrophotometric first order derivative approach for estimating Dapagliflozin and Vildagliptin in tablet dose form has been developed and validated in its tablet dosage form. The standard and sample solutions of Dapagliflozin and Vildagliptin were prepared using methanol as the solvent. Dapagliflozin was estimated at 287.2 nm and Vildagliptin at 222.9 nm for the first order derivative UV- spectrophotometric method. Beer’s law was obeyed in the concentration ranging from 5 to 15 ?g/ml for Dapagliflozin and 50 to 150 ?g/ml for Vildagliptin with coefficient of correlation value 0.998 and 0.999 respectively. The method was tested and validated for various parameters as per the ICH guidelines. The precision is expressed as relative standard deviation, which was found to be 1.58% and 0.75% respectively for the above method. The proposed method was successfully applied for the determination of Dapagliflozin and Vildagliptin in pharmaceutical formulation. Results of the analysis were validated statistically and were found to be precise. The proposed method is simple, easy to apply, low-cost and require relatively inexpensive instruments. Keywords: Dapagliflozin, Vildagliptin, first order derivative spectroscopy, methanol
Publication date: 01/03/2024

https://ijbpas.com/pdf/2024/March/MS_IJBPAS_2024_7849.pdf

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https://doi.org/10.31032/IJBPAS/2024/13.3.7849