ABSTRACT

Periodontitis is a disease that begins in the gingival tissue and progresses to the underlying tissue, altering bone homeostasis and resulting in tooth loss if left untreated. The main aim and objective of this study is to design and development of the combinational in-situ gel to treat periodontitis. Doxycycline, metronidazole and ciprofloxacin are the most widely used drugs in treating this disease belongs to three different antibiotic classes which acts against the microbes at various stages. The drug- excipient compatibility studies were done using FT-IR and determining the MIC’s using broth dilution method helps to select the triple combination as best choice to decrease the recurrence of the disease. In-situ gels of various concentrations using carbopol934 and HPMCK100 were formulated and evaluation tests such as physical appearance, pH (6.5±0.56), viscosity (29065.25±1.35 to 29815.70±1.2), gelation time (?2 min), syringeability(?1min) were performed for the developed formulations. Application of DoE (32 factorial design) helps in the enhancement of research work to determine the optimized formulation. Anti-microbial assay for the optimized formulation was carried out and largest zone of inhibition against the microorganisms was observed. From this research work, it can be assumed that the formulated triple drug anti-microbial in-situ gel was active even at lower concentrations along with prolonged release helps to decrease the dosage regimen and also decrease the recurrence of the disease. Keywords: Periodontitis, MIC, In-situ gels, sol-gel theory, pH dependent polymers, 32 factorial design & DoE
Publication date: 01/02/2024

https://ijbpas.com/pdf/2024/February/MS_IJBPAS_2024_7799.pdf

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https://doi.org/10.31032/IJBPAS/2024/13.2.7799