ABSTRACT

In the present study, synthesis of silver nanoparticles (AgNPs) using flower of Nelumbo nucifera were tested for antioxidant activity and against human breast cancer cell line (MDA-MB-231). The synthesized AgNPs were characterized using UV-visible spectroscopy, X-ray powder diffraction (XRD), fourier- transform infrared spectroscopy (FTIR), Scanning Electron Microscope (SEM) and EDAX analysis. The synthesized silver nanoparticles were checked with the colour variation and it was confirmed by UV-vis spectral analysis. The morphology of the synthesized nanoparticles were analysed using SEM. The XRD was done to find out the crystalline structure of the compound. FTIR measurements are carried out to identify the possible biomolecules responsible for capping and efficient stabilization of the silver NPs synthesized using flower of Nelumbo nucifera. The anti oxidant analysis showed better percentage inhibition for the synthesized silver nanoparticles for the DPPH (65.74?g/ml), Hydrogen peroxide (51.48 ?g/ml) and for metal chetaling (60.93 ?g/ml) at a concentration 100?g/ml. The synthesized flower-AgNPs of Nelumbo nucifera was tested against the MDA-MB-231 cell line to find out the cell viability. The synthesized silver nanoparticles using flower of Nelumbo nucifera inhibited the proliferation of human breast cancer cell line with an IC50 value of (11.25 ?g/ml). From this study, concluded that synthesized AgNPs using flower of Nelumbo nucifera have potential anti-oxidant and anticancer activity. Keywords: flower of Nelumbo nucifera, silver nanoparticles, MTT assay, MDA-MB-231 cell line, ethanol extract
Publication date: 01/02/2024

https://ijbpas.com/pdf/2024/February/MS_IJBPAS_2024_7759.pdf

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https://doi.org/10.31032/IJBPAS/2024/13.2.7759