In the current investigation, diabetic wistar rats were induced with nicotinamide-streptozotocin, and
the hydroalcoholic extract of polyherbal mixtures combinations of Ocimum tenuiflorum, syzygium
cumini and Aegel Marmelos was utilised to assess its antihyperglycemic and anti-neuropathic activities.
To test anti-diabetic activity, doses were given like standard– Metformine, PHM-1 with 200 mg/kg (low
dose), PHM-2 with 400 mg/kg (medium dose) and PHM-3 with 800 mg/kg (high dose). After the
administration for 21 days the PHM -3(low dose) was more accurate to treat diabetes and multiple
parameters including triglyceride (TG), total cholesterol (TC), SGOT, SGPT, serum creatinine, serum
bun, and uric acid are seen. To compare with other treatment groups, metformin is used. Eddy's hot
plate and hot water immersion method and cold allodynia method with the doses of polyherbal mixtures
combination of Ocimum tenuiflorum, syzygium cumini and Aegel Marmelos were given like standard-
Pregablin, PHM-1 with 200 mg/kg (low dose) and PHM-2 with 800 mg/kg (high dose) is used to assess
neuropathic action. Using the sciatic nerve ligation method, eddy’s hot plate method, hot water
immersion method and cold allodynia, neuropathic activity is assessed by the animals pain tolerance
and pain frequency after administrating the drug for 21 days the PHM-1 (High dose) shows the better
analgesic activity on rats when compared to PHM2 .The Histopathological examination confirmed the
antihyperglycemic and analgesic effect of PHM. Among the three doses PHM3 the high dose is
statistically significant with ***p<0.001 when compared to Disease control. And comparing PHM1*p<0.05 and PHM2 **p<0.01 with disease control, where as in diabetic neuropathy, Among the two
doses PHM3 is statistically significant with ***p<0.001, when comparing with disease control.
Keywords: Diabetic neuropathy, diabetes, polyherbal mixtures, tail flick method, Eddy’s hot
plate, cold allodynia method, Sciatic nerve ligation
Publication date: 01/01/2024
https://ijbpas.com/pdf/2024/January/MS_IJBPAS_2024_7736.pdf
Download PDF
https://doi.org/10.31032/IJBPAS/2024/13.1.7736
