ABSTRACT

The development and practical application of nanotechnologies have increasingly attracted the attention of researchers due to the remarkable properties that can be achieved with materials when they are on the nanometric scale of size. The goal of this study was to create zinc oxide nanoparticles (ZnO NPs), ZnO nanoparticles with 0.25%(p/p) Silver added (ZnO/Ag0.25 NPs), and ZnO nanoparticles with 0.5% (p/p) Silver added (ZnO/Ag0.5NPs) using n-hexane extract of Stephania japonica Linn. leaf, which contained phenols, flavonoids. The synthesis of the ZnO and ZnO/Ag NPs was verified by XRD and TEM, and the NPs showed a hemispherical morphology and a particle size of less than 30 nm. The NPs were produced at temperatures between 300 and 600°C. This work also aims to study the antimicrobial activity of zinc oxide nanoparticles (ZnO NPs) and Zinc Oxide and Silver matrix nanoparticles (ZnO/Ag0.25) against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) bacteria. To evaluate the antimicrobial activity of ZnO NPs and ZnO/Ag0.25, diffusion tests in solid media and Minimum Inhibitory Concentration (MIC) were performed. For ZnO NPs, the mean diameter of the inhibition halo was 1.1 ± 0.06 cm and 0.7 ± 0.15 cm for S. aureus and E. coli, respectively. The MIC of ZnO NPs determined in the present study to inhibit S. aureus ranges from 313.36?g/ml and 626.64?g, while for E. coli it was not possible to determine the MIC because the solution saturated ZnO NPs is not sufficient to effectively inhibit this bacterium. When Silver was added to ZnO NPs, it decreased their bactericidal effect against S. aureus. The findings demonstrated that the ZnO NPs produced at 300°C (average size 9.9 nm) were more effective for the in vitro control of S. aureus. As for ZnO/Ag0.25, there was no formation of inhibition halo, and it was not possible to determine the MIC for any of the bacterial strains under study, reaffirming the inability of bacterial inhibition of ZnO/Ag0.25, without a supplying source of UV and/or visible light. Keywords: Stephania Japonica Linn. leaf extract, zinc oxide, Silver, Antibacterial Activity
Publication date: 01/01/2024

https://ijbpas.com/pdf/2024/January/MS_IJBPAS_2024_7452.pdf

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https://doi.org/10.31032/IJBPAS/2024/13.1.7452