ABSTRACT

Polyhydroxyalkanoates (PHAs) may hold the answer to addressing the pollution issues brought on by petrochemical-derived plastics. Archaea represent a vast untapped source of potential for such metabolic products with interesting uses in the environment and industrial importance. Halophilic archaea have drawn a lot of attention among archaea because they are easier to handle and cultivate. Additional benefits of using haloarchaea for PHA generation include the ability to easily harvest intracellularly stored PHA from the cells by suspending them in low osmolarity solutions, such as water. This study's main objective was to check for polyhydroxy butyrate (PHB) accumulation in salt pans in the Mithapur, Surajbari (Kutch), Newport, and Nari (Bhavnagar) regions of Gujarat. 33 isolates, including extremely halophilic archaeon Haloferax alexandrines strain wsp1 isolated from Mithapur, Gujarat showed a maximum PHA accumulation of 4.37% ±0.17 of cell dry weight (CDW) at optimized conditions. The FT-IR,1H NMR and 13C NMR analysis revealed that the polymer was a co-polymer of poly(3-hydroxybutyrate-co-3-hydroxy valerate) [P(HB-co-HV)]. Keywords: polyhydroxy alkenoates (PHA), Halophilic archaea, NMR, FTIR
Publication date: 15/12/2023

https://ijbpas.com/pdf/2023/December/MS_IJBPAS_2023_DECEMBER_SPCL_1081.pdf

Download PDF
https://doi.org/10.31032/IJBPAS/2023/12.12.1081