ABSTRACT

Cyanobacteria known for their remarkable adaptability in challenging environments serve as a vital form of the study. The exploration focuses on isolation of micro algal cultures from hi-temperature habitats. The hot spring of Tuva, Gujarat through rigorous morphological and molecular phylogenetic analysis, the isolated strain were identified as Desertifilum Salkalinema. The objective is to optimize the growth rate and conditions for enhancing the efficiency of phycobiliprotein (PBP) production exhibiting its maximal growth rate at 30 ?C when cultivated in BG11 medium. Notably, Nitrogen concentration experiments within the original BG11 media by increasing the sodium nitrate concentration exhibit both growth rate and PBP as accumulated in these strains. This finding highlights the nitrogen availability and temperature on both growth dynamics and phycobiliprotein. For enhancing the PBP extraction efficiency, the mechanism identified different extortion methods. Wherein the combined extraction protocol involving freeze- through cycles plus ultrasonication exhibited a marked increase in the efficiency of phycobilin protein extraction. Finally, these observations hold significant implications for the biotechnological application of phycobilin protein, as C-PC are recognized for their antioxidant and antibacterial properties providing valuable potential of these cyanobacteria. Keywords: Phycobiliprotein, Growth, Desertifilum sp., Hot spring, Extraction mechanism
Publication date: 15/12/2023

https://ijbpas.com/pdf/2023/December/MS_IJBPAS_2023_DECEMBER_SPCL_1072.pdf

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https://doi.org/10.31032/IJBPAS/2023/12.12.1072