ABSTRACT

Background: Despite the availability of modern therapeutic interventions, diabetes mellitus (DM) remains a substantial public health concern in the 21st century. The exploration of novel phytomedicines is an increasingly expanding field of research. Objective: This study aims to conduct a preliminary screening of phytochemicals and assess the in vitro antidiabetic activity of ethanolic root extracts from Debregeasia longifolia. Materials and Methods: A standardized approach was employed in this study to investigate the materials and methods used in the research. Root extracts of Debregeasia longifolia were prepared using a soxhlet apparatus with various solvents, including ethanol, chloroform, petroleum ether, ethyl acetate, and water. The study also sought to identify different types of phytochemicals present in the roots of Debregeasia longifolia. Furthermore, the in vitro antidiabetic activity ofthese root extracts was assessed, using alpha-amylase inhibition assay and non-enzymatic glycosylation of hemoglobin as an indicator in an in vitro model. Results: The study's findings indicate the presence of phytochemicals such as carbohydrates, tannins, flavonoids, phenols, and others in Debregeasia longifolia root extracts. These extracts exhibited noteworthy inhibitory activity on alpha-amylase and non-enzymatic glycosylation of hemoglobin, suggesting their potential as antidiabetic agents. Conclusion: Based on the results obtained from this investigation, it can be concluded that Debregeasia longifolia root extracts exhibit significant antidiabetic properties in an in vitro model. Further research using an in vivo model is recommended to validate these findings. Keywords: Debregeasia longifolia, Phytochemicals, Diabetes Melitus, Antidiabetic activity
Publication date: 15/12/2023

https://ijbpas.com/pdf/2023/December/MS_IJBPAS_2023_DECEMBER_SPCL_1068.pdf

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https://doi.org/10.31032/IJBPAS/2023/12.12.1068