ABSTRACT

Microbial enzymes have unimaginable capabilities to catalyze biochemical reactions. Cholesterol oxidase is one such enzyme that converts cholesterol into cholest – 4-en-3-one, a precursor for the production of steroid hormones and pharmaceuticals. Due to its increased usage in determining blood cholesterol in laboratories and as a biocatalyst for the manufacture of many steroids, cholesterol oxidase has drawn a lot of attention. The present study aims to isolate and screen microbes from bio-dung samples for the production of cholesterol oxidase enzyme. The bacterial strain which showed maximum cholesterol oxidase activity was undergone morphological, biochemical and molecular identification and the strain was identified as Streptomyces maritimus by 16S rRNA sequencing. Streptomyces maritimus is a gram-positive bacteria belonging to Phylum Actinobacteria. Cholesterol oxidase production by Streptomyces maritimus was also optimized for various parameters. From the study, it was identified that Streptomyces maritimus was capable of producing the maximum level of cholesterol oxidase at an incubation temperature of 35ºC, pH of 6, agitation rate of 200 rpm and inoculum size of 25%. Optimum enzyme activity was observed when the medium was supplemented with galactose as carbon source, yeast extract as nitrogen source and PO42- as inorganic salt. Keywords: Cholesterol oxidase, Cholest – 4-en-3-one, Cholesterol, Streptomyces maritimus, bio dung
Publication date: 15/12/2023

https://ijbpas.com/pdf/2023/December/MS_IJBPAS_2023_DECEMBER_SPCL_1013.pdf

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https://doi.org/10.31032/IJBPAS/2023/12.12.1013