ABSTRACT

Agriculture determines the living conditions of the population of a nation. The salinity of soil affects plant growth by inhibiting the process of plant photosynthesis, protein synthesis, and lipid metabolism. According to a study by the United Nations Environment Program approximately 20 % of agricultural land and 50 % of cropland in the world is under the salt-stress. Salinization of soil converts agricultural land to barren land. The rhizobacteria showing traits to improve plant growth, can be further formulated as biofertilizers and check the plant growth by comparing various growth parameters. This study includes the extraction of bacteria from saline soil having growth-promoting properties. The sample was collected from the saline soil of Aurariya remote area in Uttar Pradesh India. The source of living for people in this area is primarily agriculture and most of the land here is saline. The isolated bacteria were tested for the PGPR traits which are the ability of IAA synthesis, Phosphate solubilization, HCN production, and ACC Deaminase activity. The strains showing PGPR traits will be molecularly characterized by 16SrRNA sequencing. The four saline soil samples were tested and the saline-tolerant bacteria were at 3%, 6%, and 9%. The ten isolates were found to be tolerant at such high salt concentrations. The bacterial isolates maximum shown the ACC Deaminase activity. IAA and HCN production test were conducted and out of 10 isolates, 5 isolates have shown the best positive results. None of the isolates were showing positive results in phosphate solubilization. The two isolates S7 & S3 showing maximum positive results for PGPR were identified by 16SrRNA sequencing and were identified; S7 Bacillus cereus and S3 closely resembled Bacillus licheniformis and Bacillus subtilis. The native Microorganisms in saline soil have the inherent properties to tolerate saline conditions and interact with the plants helping in their growth, by synthesizing compatible solutes and production of plant growth- promoting hormones. The isolation of novel microorganisms may provide an effective way to deal with the problem of salinity. Thus, the competent microorganism can be used as biofertilizers and their positive effects can be spread to farmers to promote sustainable agriculture. Keywords: Agriculture, Biofertilizer, Environment, Eco-friendly, Ecosystem, HCN, PGPR, Salinity, Soil
Publication date: 15/12/2023

https://ijbpas.com/pdf/2023/December/MS_IJBPAS_2023_DECEMBER_SPCL_1010.pdf

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https://doi.org/10.31032/IJBPAS/2023/12.12.1010