ABSTRACT

To understand the pathogenesis, diagnosis, treatment, and prevention of tuberculosis (TB), Mycobacterium tuberculosis (MTB) needs to be molecularly characterized. There is, however, a paucity of knowledge regarding the molecular traits and drug-resistant patterns of MTB in individuals with pulmonary and extra pulmonary tuberculosis. The objective of this study was to identify the levels and patterns of resistance of MTB isolates to two important anti-TB medications, rifampicin and isoniazid, as well as the types of mutations in the isolates' drug resistance genes, rpoB, katG, and inhA. This retrospective analysis aimed to comprehend and record our experience using Line probe assay (LPA) for M. tuberculosis complex detection and MDR-TB diagnosis under programmatic circumstances. 317 samples from individuals with a variety of presentations were analyzed overall in the current study. A direct smear with Acid Fast Bacilli was made for the samples. Using the Fluorescent Microscopy procedure, all of the smears were stained. 206 samples had favourable results from smear microscopy examination. LPAs showed overall high accuracy for the identification of (Rifampicin) RIF resistance in patients with pulmonary and extrapulmonary tuberculosis. LPAs showed good sensitivity and high specificity for detecting INH resistance. When applied to smear-positive specimens, LPAs have a high levelof accuracy for the detection of M. tuberculosis. For the detection of MTB and drug-resistant TB, particularly RIF-resistant, INH-resistant, and MDR-TB, LPAs have good diagnostic performance. Keywords: Mycobacterium tuberculosis, RIF-resistant, INH-resistant, MDR-TB and Line probe assay
Publication date: 15/12/2023

https://ijbpas.com/pdf/2023/December/MS_IJBPAS_2023_DECEMBER_SPCL_1001.pdf

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https://doi.org/10.31032/IJBPAS/2023/12.12.1001