ABSTRACT

HPLC is an analytical method and key tool for the drug separation, drug quality, monitoring of drugs and in the research of life science. The analysis and separation of non-volatile compounds are majorly done by HPLC method. HPLC deals with variety of applications like high sensitivity, accuracy, precision and resolution. The retention time in the RP-HPLC is calculated fewer than 100% in the aqueous condition. The aim of the work was to develop RP-HPLC method for combination of drug and it is validated as per ICH guidelines My whole work is to discuss the analytical method development, validation and optimization of alfacalcidol by RP- HPLC developed for the determination of osteoporosis and it stimulates intestinal calcium absorption with the selection of mobile phase, review of literature, selection of chromatographic conditions, wavelength and detectors used for the quantification of combination of drug which will be useful for the research scholars for development and optimization of drug combination Keywords: Method development, validation, RP-HPLC, application, quantification
Publication date: 01/12/2023

https://ijbpas.com/pdf/2023/December/MS_IJBPAS_2023_7645.pdf

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https://doi.org/10.31032/IJBPAS/2023/12.12.7645