ABSTRACT

The Bioanalytical Method was Developed and Validated for the Simultaneous Estimation of Remogliflozin Etabonate and Vildagliptin in Tablet formulation. Both drug was Extracted by Protein Precipitation Method. Internal standard used as a Metformin. The estimation was achieved on the C18 column with Isocratic mixture Phosphate Buffer: Acetonitrile pH 3 adjusted with 0.1 % Orthophosphoric acid in the ratio of 30:70 v/v was used as Mobile Phase at flow rate 0f 1.0 ml/min. injection volume 20?l. and wavelength of detection was kept at 229 nm. The Retention time of Remogliflozin was 3.1 min and Vildagliptin was 6.1 min. Metformin 1.5 mint. The Linearity of the proposed method was investigated in the range of 10 ?g/ml- 160 ?g/ml for the formulation. The correlation coefficient for Remogliflozin was 0.999
Publication date: 01/12/2023

https://ijbpas.com/pdf/2023/December/MS_IJBPAS_2023_7635.pdf

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https://doi.org/10.31032/IJBPAS/2023/12.12.7635