ABSTRACT

Antibiotic resistance is a critical public health issue that affects people all over the world. Bacteriophage (phage) therapy, as an alternative to antibiotics, is one of the most effective ways to combat antibiotic resistance. For phage therapy bacteriophage must be both isolated from the environment and show lytic activity against target bacterial pathogens. A single phage or a mixture of phages can also be employed in phage therapy; mixed phages are more successful than single phages at lowering the number and/or activity of harmful bacteria. we aimed to use mixed phages against three AMR bacteria (Pseudomonas aeruginosa, Escherichia coli, and Serratia marcescens). Frequently first isolation of phages is done and then characterization, to isolate the phage alteration of the procedure is possible to get the desirable results. Isolated mixed phages were able to completely lyse bacterial cells and they are effective on all three AMR bacteria. These isolated mixed phages may be a good substitution to bacteria that are resistant to antibiotics and can also use in phage therapy. Keywords: Bacteriophage isolation, phage therapy, AMR bacteria, antimicrobial activity
Publication date: 01/12/2023

https://ijbpas.com/pdf/2023/December/MS_IJBPAS_2023_7632.pdf

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https://doi.org/10.31032/IJBPAS/2023/12.12.7632