ABSTRACT

The aim of this research work was to develop a simple, accurate, sensitive and validated Ultra Violet (UV) spectrophotometric assay using multivariate regression method for the analysis of Donepezil. This multivariate calibration technique was based on equations constructed using linear regression analysis using the correlation between absorbance and concentration at five selected equidistant wavelengths. Donepezil had a maximum wavelength of 220 nm and 263 nm. The findings were statistically analysed for significance. A linear plot in the concentration range of 7-13 ?g/mL, with a regression co-efficient of 0.999 was obtained. The % RSD for intra-day and inter-day precision were 0.3162, 0.3145 and 1.0416, 0.6892 respectively. The assay was determined and found to be 98.13% - 101.50 % w/w. Keywords: Donepezil, Anti Alzheimer drug, UV spectrophotometry, Multivariate calibration, Assay, ICH guidelines
Publication date: 01/12/2023

https://ijbpas.com/pdf/2023/December/MS_IJBPAS_2023_7620.pdf

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https://doi.org/10.31032/IJBPAS/2023/12.12.7620