ABSTRACT

The aim of present investigation is to establish simple, precise, economical, rapid, and accurate first order (D1) UV-Spectrophotometric method for simultaneous estimation of Fluconazole (FCZ) and Quercetin (QCT) from developed mucoadhesive In-situ vaginal gel formulation. The method was developed in Simulated Vaginal Fluid (SVF) at two different pH, normal vaginal pH (4.31) and in Infected vaginal pH (7.00). In developed fist order UV- spectrophotometric method, FCZ was quantify at detection wavelength, 232.03 nm, and 268.03 nm in SVF, pH 4.31 and pH 7.00, respectively. While QCT can easily quantified at same detection wavelength, 400 nm in both SVF, pH 4.31 and pH 7.00. The method was also validated as per ICHQ2(R1) guidelines. The scope of developed method includes quantification of FCZ and QCT from its developed mucoadhesive In-situ vaginal gel formulation and identification of drug released profile of both analytes in both normal vaginal pH (4.31) and infected vaginal pH (7.00). Keywords: Simultaneous estimation, Fluconazole (FCZ), Quercetin (QCT), First order (D1) UV-Spectrophotometric method, ICH Q2(R1) Guideline
Publication date: 01/12/2023

https://ijbpas.com/pdf/2023/December/MS_IJBPAS_2023_7617.pdf

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https://doi.org/10.31032/IJBPAS/2023/12.12.7617