ABSTRACT

Objective: The research was focused to fabricate and assess ciprofloxacin loaded microspheres and subsequent impregnation of microspheres into collagen based 3-D scaffolds for controlled delivery. Methods: Ciprofloxacin chitosan microspheres were developed by ionic precipitation and chemical cross-linking method later characterized for particle size, surface morphology, drug entrapment efficiency and in-vitro drug release. Adjacently, collagen (type-I) was extracted from bovine tendons and characterized for hydroxyproline content, circular dichromism and electrophoresis methods. The optimized microspheres were impregnated in 1% collagen and fabricated into a three dimensional scaffold by lyophilization. Similarly the microspheres were also impregnated into chitosan and PVA scaffold of 1% each for comparison of drug release patterns. The scaffolds were characterized for morphology, pore size distribution,swelling, chemical incompatibility, thermal behavior, in-vitro drug release and anti-bacterial (collagen scaffolds) efficiency. Results: The average particle size of microspheres were found to be 229 ?m with porous surface texture, entrapment efficiency of 67.66% and controlled drug release (98.04%, 14h). The collagen extracted was found to be of good purity. All the scaffolds showed good morphology with even pore size distribution, acceptable swelling index and thermal stability. First-order release with initial burst release of drug from collagen scaffold was noticed and followed by controlled release over several hours. Conclusion: The collagen scaffolds containing microspheres exhibited its supremacy over other scaffolds in all parameters and effective against selected bacterial strains. Thus, collagen based scaffold loaded with microspheres containing antibacterial agent serves as a promising biomaterial in biomedical applications. Keywords: Ciprofloxacin, collagen, ionic-gelation, lyophilization, microspheres, scaffolds
Publication date: 01/12/2023

https://ijbpas.com/pdf/2023/December/MS_IJBPAS_2023_7612.pdf

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https://doi.org/10.31032/IJBPAS/2023/12.12.7612