ABSTRACT

The present work proposes determining Gemifloxacin in bulk and formulations by developing a novel approach and verifying. It is an analytical technique that is sensitive, exact, and rapid. Using a UV spectrophotometer and multivariate calibration technique, the analytical method was carried out. The relation between concentration and absorbance, which has been determined at five different wavelengths, including the absorption maxima, was used to build this multivariate calibration technique. The linear regression equations, and other mathematical and statistical techniques were used to validate the process in accordance with ICH Q2 (R1) criteria. Precision, accuracy, linearity, and other validation parameters has listed in the instructions has been satisfied. The relationship between concentration and absorbance was linear for concentration levels between 3.5 and 6.5 ?g mL-1. This method is cost effective and has good sensitivity, and produces good findings when employed in regular analysis of drugs and formulations. The method greenness values were calculated using the analytical Eco scale, Agree metrics, and Green Analytical Procedure Index. Keywords: Gemifloxacin, Multivariate calibration technique, UV Spectrophotometer, Pharmaceutical formulations, ICH guidelines, Validation
Publication date: 01/11/2023

https://ijbpas.com/pdf/2023/November/MS_IJBPAS_2023_7586.pdf

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https://doi.org/10.31032/IJBPAS/2023/12.11.7586