ABSTRACT

A novel, simple method was developed to evaluate Abacavir, Dolutegravir, and Lamivudinein bulk and formulation by RP-HPLC. The principle analytes were eluted with the conditions of the mobile phase having the Ethanol: Ethyl acetate (80:20, % v/v) using the Lichrosphere RP C8 column (Phenomenex, USA (250 x 4.6 mm, 5?) analytical column with the 1.0 ml/min flow rate and 10?l injection volume at 260 nm in UV detector. The retention times of Abacavir, Dolutegravir, and Lamivudine were 2.31min, 3.120 min, and 4.59min with a total run time of 6 min. The curve indicates correlation coefficient (r2) was superior by having a value nearer to 1.000 with a linear range of 40 ?g/ml-130.0 ?g/ml for Abacavir, Dolutegravir, and Lamivudine. The correlation coefficient (r2) 0.9971for Abacavir, 0.9979 for Dolutegravir and 0.9947 for Lamivudine were found. The LOD and LOQ for the Abacavir, Dolutegravir, and Lamivudine were found at 1.40 ?g/ml, 3.01?g/ml, 5.84?g/ml, and 4.25 ?g/ml, 9.12 ?g/ml and 17.71 ?g/ml. The developed method was applied for the bulk and formulation in routine analysis. Keywords: Abacavir, Dolutegravir, Lamivudine, HPLC
Publication date: 01/11/2023

https://ijbpas.com/pdf/2023/November/MS_IJBPAS_2023_7545.pdf

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https://doi.org/10.31032/IJBPAS/2023/12.11.7545