ABSTRACT

Objective: The aim of this study was to investigate the In vitro antioxidant activity and nephroprotective activity of the extract of Pithecellobium dulce bark. Methods: The Pithecellobium dulce bark was extracted using a solvent such as methanol and water. The antioxidant activity of these extracts was evaluated by using different assay such as 2,2-diphenyl-1-picrylhydrazyl (DPPH) method, scavenging activity of superoxide anion and nitric oxide (NO) scavenging assay and in vitro nephroprotective screening of various concentrations of methanol and aqueous extract of bark were tested cytoprotective and epifluorescence staining assay was performed against normal kidney cell treated with gentamicin. Results: The phytochemical study indicate the presence of phenols, tannin, flavonoids and steroid in the methanolic and aqueous extracts of the plant. Bark extracts clearly revealed the dose-dependent increase in nephroprotection. The presence of phytoconstituents in Pithecellobium dulce was inhibited the induced nephrotoxicity. Antioxidant activity results indicated that extracts were exhibited the ability to quench the DPPH radical, good antioxidant potential with free radical scavenging activity. The methanol and aqueous extracts at different concentration the prevent the toxicity effect to cell in place of inducer. According to IC50, both extracts showed more than 100 so its non-toxic to cell as compared togentamicin itself. live cells and dead cells can be differentiated by epifluorescence staining. Both the extracts showed nontoxic on kidney cell. The maximum NO scavenging capacity was found in methanol extract with an IC50 value of which was more than water extract. Conclusion: Phytochemical screening indicated the presences of steroids, saponins, flavonoids, tannins and phenolic compounds in methanol and aqueous extracts of P. Dulce. Our study also proved that this drug possesses considerable amount of flavonoid, phenolic compounds and tannin. In vitro antioxidant and cytotoxicity studies illustrated that P. dulce possessed significant free radical scavenging activity. Keywords: Pithecellobium dulce, Phenols, Tannin, Flavonoids, Antioxidant, DPPH, Epifluorescence, Cytotoxicity
Publication date: 01/11/2023

https://ijbpas.com/pdf/2023/November/MS_IJBPAS_2023_7536.pdf

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https://doi.org/10.31032/IJBPAS/2023/12.11.7536