ABSTRACT

Bilirubin is primarily produced in the liver when hemoglobin is broken down. Understanding the bilirubin concentration in serum is essential for assessing liver health and diagnosing hyperbilirubinemia (which affects 60% of full-term & 80% of preterm infants). Potentiometry based on (ISEs) has undergone an evolution due to advancements in the sensitivity & selectivity values of ISEs, the incorporation of latest elements, novel recognizing approaches (from conventional potentiometry to dynamic electrochemistry technique), & a greater theoretical comprehension & prediction of ISE potentiometric sensitivities. The sensor's polymeric ion-selective membrane monitors only free ionic bilirubin (also known as "unbound" BR, or BR that is not bound to albumin perhaps additional entities of complexity), regardless of the existence of more anions in serum, such as chloride, phosphate, pyruvate, deoxycholate, & lactate. When bilirubin is detected in a pH of 8.4 sodium phosphate buffer, the linear response of the sensor encompasses bilirubin levels in serum that is clinically significant. This potentiometric biosensor was successful in detecting free BR in human serum. The created potentiometric sensor is a potential instrument for the fast, precise, & targeted analysis of blood serum-free bilirubin, which may have clinical diagnostic applications Keywords: Bilirubin, Jaundice, Biosensors, Potentiometry, Free Bilirubin, Ion Selective Electrode, Hyperbilirubinemia
Publication date: 15/10/2023

https://ijbpas.com/pdf/2023/October/MS_IJBPAS_2023_OCTOBER_SPCL_1053.pdf

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https://doi.org/10.31032/IJBPAS/2023/12.10.1053