ABSTRACT

Arogyavardhini Vati & Livamrit Advance (Livamrit A) is a Polyherbal formulation that is being used for centuries with claimed efficacy and safety in the treatment of Jaundice, liver disease, and various skin conditions. Considering their use by a larger population in India they must be assessed for their efficacy and cytotoxic effects, if caused any therefore the present study was aimed to evaluate the physicochemical and cytotoxicity effect of Arogyavardhini Vati and Livamrit tablets.Both Ayurvedic formulations were evaluated for their physicochemical properties by AAS, phase analysis through XRD, and cytotoxicity through MTT assay on HepG2 cell line in five different concentrations (62.5?g/ml, 125 ?g/ml, 250 ?g/ml, 500 ?g/ml, and 1000 ?g/ml) of the drugs.The Physicochemical analysis resulted in a very low concentration, below the permissible limit of targeted heavy metals i.e. Cadmium, Lead, Zinc, and Copper in the tested samples. The phase analysis revealed crystalline structure in Arogyavardhini Vati in two different phases of their active ingredients only namely Metacinnabar (HgS) and Hematite (Fe2O3) while Livamrit A did not show any crystalline structure. Also, not any significant change in cell viability was observed in the exposure to higher dose concentrations (1000 ?g/ml) of both formulations.In our study both Ayurvedic formulations (Arogyavardhini Vati & Livamrit A) are found safe having no heavy metals in higher concentrations than the permissible limit and any considerable cytotoxic effects on the liver. Keywords; HepG2, Ayurvedic Formulations, Cytotoxicity assessment of Ayurvedic formulations, Heavy metals toxicity, MTT Assay, Arogyavardhini Vati, Livamrit A
Publication date: 01/10/2023

https://ijbpas.com/pdf/2023/October/MS_IJBPAS_2023_7511.pdf

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https://doi.org/10.31032/IJBPAS/2023/12.10.7511