ABSTRACT

Bioanalysis is the term utilized to depict the quantitative assessment of drugs / metabolite in biological fluids. LC-MS combines the chromatographic separation and mass analysis capabilities. Loperamide in human plasma were validated using UPLC-MS/MS technique. Loperamide is the drug which is mainly used for the treatment of sudden diarrhea. Internal standard used in this method is Fluconazole and 5 mM Ammonium acetate is used as buffer. Acetone-M, Formic acid, and 5mM Ammonium acetate (80:20:01%v/v/v) is used as solvent. ZORBAX Eclipse XDB-C8(100mm × 4.6mm, 3.5?m) column used. The bio-analytical method SOP No.MTR-BA-M-452-00, Loperamide in K2EDTA human plasma in the concentration range 24.8560-5063.9260pg/ml was successfully developed and validated. Keywords: Bio-analytical method, Loperamide, Fluconazole, UPLC, Human plasma, Calibration curve
Publication date: 01/10/2023

https://ijbpas.com/pdf/2023/October/MS_IJBPAS_2023_7460.pdf

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https://doi.org/10.31032/IJBPAS/2023/12.10.7460