ABSTRACT

Angiogenesis, the expansion of a capillary network from preexisting vasculature, is implicated in a number of pathological conditions. Now a day’s stimulation or suppression of angiogenesis process is being explored as therapy. Conversely, therapeutic angiogenesis contributes to regulate various pathological conditions. This study is scientifically designed to observe the anti-angiogenic potential of hydro alcoholic extract of seeds of Raphanus sativus L. with Chicken Chorioallantoic Membrane (CAM) assay. In ovo chicken CAM assay was used to examine the anti angiogenic activity of hydro alcoholic extract of R.sativus seeds (HERS). After sufficient embryonic development treatment of extract was given on CAM of fertile han eggs. Quantitation of blood vessel was assessed by photo micrographic observation and analyzed for all treatments and controls. Study revealed that photomicrograph of different chick CAMs after 72hrs of treatment with control and with different concentration of HERS at a dose of 10, 20, 30 mg/ml could significantly inhibit HIF-1? and VEGF induced angiogenesis in in-ovo chicken CAMs. This concurred with manual quantitation of tertiary as well as quaternary blood vessels, which indicated that HERS suppress the formation of new blood vessels as compared to control. HERS is found to produce a dose dependent anti angiogenic activity in CAM assay. This effect may be postulated due to the presence of phytochemicals like sulforaphane & sulphoraphene, gallicacid, caffeic acid, quercetin and apigenin which is reported to have potential anti-angiogenic activity by modulating multiple signaling cascades. Keywords: Angiogenesis, CAM Assay, Raphanus sativus L., sulphoraphene; VEGF
Publication date: 01/10/2023

https://ijbpas.com/pdf/2023/October/MS_IJBPAS_2023_7449.pdf

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https://doi.org/10.31032/IJBPAS/2023/12.10.7449