ABSTRACT

Medicinal plants hold a very important place in everyday life. By their bio- availability, the population tends to use them to treat different diseases. The aim of this research is to know the different phytochemical compounds found in the different extracts of foliage and bark of Rauvolfia media followed by a quantitative estimation of the major phytoconstituent present in the extracts and the evaluation of the antioxidant activity. The present investigation includes a phytochemical screening of the barks and leaves extracts, followed by a quantitative analysis of the extracts where the total flavonoid and phenolic content of R. media part were evaluated by aluminum chloride and Folin-Ciocalteu respectively, and finally the evaluation of the scavenging effect by 1,1-diphenyl, 2-picryl hydrazyl (DPPH) assay. The screening results showed that alkaloids, saponins, tannins, phenols, and flavonoids are present in all fractions. The quantitative analysis conveyed that the highest concentration of phenols was found in the barks with 5.208 ± 0.15 mg GAE/g, while the highest levels of flavonoids in the leaves with 15.868 ± 0.05 mg QE/g. The methanolic fraction of the leaves possessed the highest percentage scavenging activity with an IC50 of 1.038 ?g/ml compared with ascorbic acid, used as standard (1.057 ?g/ml), at a concentration of 120 ?g/ml. Keywords: Rauvolfia media, screening, Phytochemical screening, Antioxidant activity
Publication date: 01/10/2023

https://ijbpas.com/pdf/2023/October/MS_IJBPAS_2023_7445.pdf

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https://doi.org/10.31032/IJBPAS/2023/12.10.7445