ABSTRACT

The present study reported the bioactivity-guided isolation of biologically active curcumin from a widely used Zingiberaceae ethnomedicinal plant Curcuma caesia Roxb from the Upper Brahmaputra Valley Zone of Assam. A systematic approach that includes TLC – Bioautography (TLC-BA), column chromatography (CC), and preparative TLC (PTLC) techniques were employed in order to isolate and purify the potent biologically active compound from the rhizome extract of C. caesia. The crude ethyl acetate rhizome extract of C. caesia was partially purified through thin-layer chromatography (TLC) and bioautography. The positive band showing the highest antimicrobial activities from TLC was subjected to silica gel column chromatography for further purification. The isolated compound was analyzed for spectral studies like- GC-MS, LC-MS, FT-IR, 1H NMR, and 13C NMR for structural elucidation. The purified compound in C. caesia was chemically characterized as Curcumin, a curcuminoid having potent antimicrobial activity. Curcumin and its derivatives are considered potent therapeutic agents having antimicrobial, anticancer, anti- inflammatory, antitumor, anti-acidogenic, wound healing, and anti-oxidant activities. Key words: C. caesia, TLC, Bioautography, Curcumin
Publication date: 01/09/2023

https://ijbpas.com/pdf/2023/September/MS_IJBPAS_2023_7429.pdf

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https://doi.org/10.31032/IJBPAS/2023/12.9.7429