ABSTRACT

To investigate the therapeutic effects of Theriophonum sivaganganum methanolic extract and its fractions for ?-amylase, ?-glucosidase and ?-galactosidase inhibitory activity by in vitro assays. Tubers of Theriophonum sivaganganum (TS) were extracted with methanol by maceration. And its fractions were prepared by dispersing uniformly in distilled water and subjected to solvent fractionation with toluene, ethyl acetate and butanol. The fractions were concentrated under reduced pressure. The inhibitory effect of these TS methanolic extract and its fractions on ?-amylase, ?-glucosidase and ?-galactosidase enzymes was determined by in- vitro assays. The results revealed that the methanolic extract and its fractions of Theriophonum sivagnganum were inhibited ?-amylase, ?-glucosidase and ?-galactosidase enzymes in a dose dependent manner. Among all these ethyl acetate fraction of Theriophonum sivagnganum has shown the prominent ?-amylase, ?-glucosidase and ?-galactosidase enzyme inhibitory activity with IC50 values of 5.75±0.18, 5.64±0.74 and 10.86±0.13mg/ml and it was well comparable with standard IC50 value 5.29±0.59, 5.15±0.48 and 10.16±0.57mg/ml respectively. And Phytochemical reports of TS revealed that it contains alkaloids, carbohydrates, steroids, saponins, flavonoids and phenolic compounds. These results substantiate the use of Theriophonum sivaganganum as medicine for the treatment of diabetes by controlling postprandial hyperglycemia. The results suggest that ethyl acetate fraction of tubers of Theriophonum sivaganganum shown significant ?-amylase, ?-glucosidase and ?-galactosidase enzyme inhibitory activity, which may be helpful to reduce the postprandial blood glucose levels. However, the chemical compounds responsible for the inhibitory action of these enzymes need to be further identified and characterized. This may be useful for the development of new antidiabetic agents from plant sources. Keywords: Theriophonum sivaganganum, ?-Amylase, ?-Glucosidase AND ?-Galactosidase
Publication date: 01/09/2023

https://ijbpas.com/pdf/2023/September/MS_IJBPAS_2023_7396.pdf

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https://doi.org/10.31032/IJBPAS/2023/12.9.7396