ABSTRACT

Banana is used widely consumed because of its nutritional values. Banana peel is a part of banana fruit that also has the antioxidant and antibacterial activity against microorganisms but has not been studied extensively. There are few studies that relate the antioxidant status and antibacterial activity of essential oil from banana peel against common human pathogens. This study aimed to evaluate the antioxidant and antibacterial activity of peel of Musa, acuminate L, dwarf cavendish banana in an in vitro experimental set-up. The fruits of M. acuminate L (dwarf cavendish banana) were obtained from local market. Standard strains of Escherichia coli and Staphylococcus aureus were used in this study which were obtained from the National Centre For Cell Science, Pune, India. The antioxidant activities of the extracts were evaluated by using the Diphenyl-1- picrylhydrazyl (DPPH) free radical scavenging method. While, antimicrobial activities of the extracts and isolated components were evaluated using Minimum Inhibition Concentration (MIC). The essential oils obtained from hexane and chloroform extracts displayed high antimicrobial and antioxidant activity. Antioxidant activity of chloroform extracts was comparable to those of synthetic antioxidants such as butylated hydroxyanisole and butylated hydroxytoluene. Among all isolated components ß-sitosterol, malic acid, succinic acid, palmitic acid, 12-hydroxystrearic acid, glycoside, the d-malic and 12-hydroxystrearic acid were the most active against both Escherichia coli and Staphylococcus aureus species tested. The MIC of d-malic and succinic acid was varying between 140- 750 ppm, respectively. Essential oils extracted from peel extracts of M. acuminate L (dwarf cavendish banana) were able to scavenge free radicals, hence can be employed as an alternative source of medicine to mitigate the diseases associated with oxidative stress. Further, the essential oils extracted from peel extracts of M. acuminate L (dwarf cavendish banana) could be considered as a good antibacterial agent against both Escherichia coli and Staphylococcus aureus to replace the synthetic medicines in treatment of diseases caused by these bacteria. Keywords: Banana, Escherichia coli and Staphylococcus aureus, Essential oils, antioxidant
Publication date: 01/09/2023

https://ijbpas.com/pdf/2023/September/MS_IJBPAS_2023_7367.pdf

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https://doi.org/10.31032/IJBPAS/2023/12.9.7367