ABSTRACT

Cellulases are a family of hydrolytic enzymes that may breakdown the organic polymer, cellulose to smaller constituents like glucose subunits. Cellulases have a wide range of applications in pulp, paper, textile, laundry, food and animal feed industries. The current study was executed with an objective to screen out cellulolytic ability of fungi isolated from degrading litter samples collected from Orchha wildlife sanctuary, M.P. Firstly, isolation of fungi has been carried out on Potato dextrose agar media then after, cellulolytic activities of isolated fungi were screened out on Czapek’s Dox agar medium having 1% carboxymethyl cellulose as substrate. Fungal colonies were stained with 0.1% congo red dye, then observation for the appearance of clear zone was done. Thirty two fungal species were used for the present study of which twenty seven were recorded as positive for cellulolytic activity. The maximum activity was achieved by Rhizopus stolonifer, Penicillium decumbens, Penicillium chrysogenum with cellulolytic index 1.66, 1.6 and 1.44 respectively. Keywords: Cellulases, Litter, Fungi, Potato dextrose agar
Publication date: 01/09/2023

https://ijbpas.com/pdf/2023/September/MS_IJBPAS_2023_7301.pdf

Download PDF
https://doi.org/10.31032/IJBPAS/2023/12.9.7301