ABSTRACT

The aim and objectives of the research are to formulate and evaluate the cosmeceutical anti-aging night lotion of Madhuca longifolia (ML) extract. To determine the efficacy of the extracts by their quantitative estimation of total polyphenolics and flavonoids content, and in vitro DPPH antioxidant potential. The fatty acid components in petroleum ether extracts were separated on the TR-FAME capillary column of gas chromatography and effluent monitored by a flame ionization detector (FID). Total phenolic and flavonoid content were determined by Folin Ciocalteu and aluminum chloride reagent respectively. Antioxidant activity is determined by the DPPH assay. The anti-aging lotion containing petroleum ether and hydroalcoholic extracts of ML seeds was formulated by o/w type of emulsion. Gas chromatography revealed the presence of oleic acid, palmitic acid, stearic acid, and linoleic acid. The total phenolic and flavonoid content was found to be 43.9 ± 0.13mg/g GAE and 41.7 ± 0.125 mg/g equivalent to naringenin. DPPH assay of the hydroalcoholic extract showed IC50 value 43.419 ?g/ml. The optimized formulations batch F3 & F4 complies with evaluation parameters like viscosity, spreadability, and are devoid of any irritation, microbial growth. Anti-aging night lotion showed good hydration of the stratum corneum after a month of application. The night lotion follows the ICH guideline of stability shown at a temperature of 400C±20C with75±5% RH for 3 months. The phytoconstituents in ML extracts elicit antioxidant activity due to high flavonoid content. ML extract loaded w/o emulsion lotion revealed its effectiveness as an anti-aging night lotion. Keywords: Madhuca longifolia, Cosmeceutical, Anti-aging, Night lotion, Antioxidant, DPPH assay
Publication date: 01/09/2023

https://ijbpas.com/pdf/2023/September/MS_IJBPAS_2023_7283.pdf

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https://doi.org/10.31032/IJBPAS/2023/12.9.7283