ABSTRACT

Objectives: The present study was investigated to evaluate antiproliferative potential of the chemopreventive potential of Mosinone-A on cell proliferation, apoptosis and cell cycle proteins during 7, 12-dimethylbenz[a]anthracene (DMBA) induced hamster buccal pouch (HBP) carcinogenesis. Materials and methods: A total number of 40 golden Syrian hamsters were randomized into 4 groups of 10 animals in each. Group I animals were served as untreated control. Groups II and III animals were painted with 0.5% DMBA in liquid paraffin three times per week for 14 weeks on the left buccal pouches. Group III animals were orally administered with Mosinone-A (2 mg kg-1 b.wt) starting one week before the exposure to the carcinogen and continued on days alternate to DMBA painting, until the sacrification of the animals. Groups IV animals were received Mosinone-A alone throughout the experimental period. Results: Topical application of DMBA for 14 weeks induced buccal pouch carcinomas associated with increased expression of, P21, whereas decreased expression of Bax, Cleaved caspase-9. Oral administration of Mosinone-A significantly inhibited the development of HBP carcinomas as revealed by decreased expression of P21, and over expression Bax, Cleaved caspase-9, Conclusion: The result of the present study indicates that Mosinone-A can exerts protective effects against DMBA induced buccal pouch carcinogenesis. These findings suggest that Mosinone-A exerts its anticancer properties by inhibiting cell proliferation and inducing differentiation and apoptosis. Keywords: Oral cancer; Mosinone-A; DMBA; apoptosis; Westernblot
Publication date: 01/09/2023

https://ijbpas.com/pdf/2023/September/MS_IJBPAS_2022_6552.pdf

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https://doi.org/10.31032/IJBPAS/2023/12.9.6552