ABSTRACT

BACKGROUND: Caffeine is consumed daily by humans and people tend to miss out on its medicinal properties such as inhibition of bacterial growth and micro-organisms. There are less studies conducted to confirm its medicinal properties overall. In most of the countries the daily consumption of caffeine is high. Caffeine has proven to have some microbial activity as per other studies. Hence more studies can be conducted targeting specific oral pathogens and using caffeine to see the antimicrobial, antibacterial and other key properties. Studies have been conducted with caffeine to treat periodontal and gingival disease. OBJECTIVE: The aim of this study was to determine the antimicrobial activity of caffeine against streptococcus mutans. MATERIALS AND METHODS: Caffeine was extracted from roasted coffee beans and was sterilised by an autoclave and stored. 1ml from the sample autoclaved was taken in 3 cuvettes. 1 cuvette being the control (saline). McFarland standard was used to adjust the turbidity of bacterial suspension. 10 ml of S.mutans was added to each cuvette, from the cuvette it was added to the culture plate (Manitol Salt agar). After 5 minutes, the culture plate was incubated at optimum temperature. After 24 hours the culture plates inoculated with S.mutans and caffeine extract were examined and colonies formed were counted. RESULTS: Antimicrobial activity by caffeine was observed with the increase in concentration and the antimicrobial activity was minimal in lower concentrations. CONCLUSION: Caffeine extract has shown antimicrobial activity against streptococcus mutans. It was confirmed after reading the culture plates for CFU (Colony forming units). Keywords: Caffeine, antimicrobial activity, streptococcus mutans
Publication date: 01/08/2023

https://ijbpas.com/pdf/2023/August/MS_IJBPAS_2023_7386.pdf

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https://doi.org/10.31032/IJBPAS/2023/12.8.7386