ABSTRACT

Blacktail Snapper (L. fulvus) muscle obtained from by-catch resources was used as an alternative source for mammalian collagen. The aim of the study was to isolate the Acid soluble collagen (ASC) and Pepsin soluble collagen (PSC) from body muscles of Blacktail Snapper (L. fulvus) . Body muscles of Blacktail Snapper (L. fulvus) the exoskeleton was removed, the muscles were cut into small pieces (0.3-0.5 cm) and stored at -4°C until used. Blacktail Snapper (L. fulvus) was extracted with 0.1M NaOH to remove non-collagenous protein for 3 days. Then, the deproteinised muscles was washed with distilled water and lyophilized for further analysis. The net yield of ASC and PSC was provisionally estimated as 28% and 47% respectively in dry weight basis. The molecular masses of the ASC and PSC subunits (?1, ?2) were about 67 kDa and 63 kDa. These finding shows the great potential of Blacktail Snapper (L. fulvus) muscle collagen as a new source biomedical materials, food and nutraceutical industries. Keywords: Blacktail Snapper (L. fulvus) Collagen; SDS-PAGE; FT-IR spectroscopy. Acid solublised collagen (ASC), Pepsin solubilised collagen (PSC)
Publication date: 01/08/2023

https://ijbpas.com/pdf/2023/August/MS_IJBPAS_2023_7380.pdf

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https://doi.org/10.31032/IJBPAS/2023/12.8.7380