ABSTRACT

Biological fluids like blood, serum, urine, tissue extracts or plasma may contain compounds like drugs and their metabolite, and Bio analysis is the term which is commonly used for the purpose of quantitative determination of this compound. An accurate Liquid chromatography with tandem mass spectrometry technique to quantify Norethindrone in dipotassium ethylene diamine tetra acetic acid human plasma over the concentration range 0.1660 ng/mL to 34.594 ng/mL as developed and validated. Norethindrone becomes extracted from plasma by means of the usage of solid Phase extraction method turned into accomplished on Acquity Ultra performance liquid chromatography ethylene bridged hybrid C18 (2.1 mm x 100 mm, 1.7 ?m) column. This method is commonly used for sample analysis to support bio-equivalence/ bioavailability and/or pharmacokinetic studies involving formulations of Norethindrone. Keywords: Bio-analytical method, Norethindrone, Ultra performance liquid chromatography- tandem mass spectrometry, Human plasma, Standard deviation, Lower and upper limit of Quantification
Publication date: 01/08/2023

https://ijbpas.com/pdf/2023/August/MS_IJBPAS_2023_7376.pdf

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https://doi.org/10.31032/IJBPAS/2023/12.8.7376