ABSTRACT

In India, Non-Communicable Diseases were estimated to account for 63% of all deaths, and cancer (CA) was one of the leading causes (9%). Cervical cancer is the fourth most frequent cancer in women with an estimated 604,000 new cases in 2020. Of the estimated 342,000 deaths from cervical cancer in 2020, about 90% of these deaths occur in low- and middle-income countries. In this In-vitro study, the anti-cancer effect on the HeLa cell line was evaluated. Cancer cells were exposed to various concentrations of the Siddha formulation Vithu Rasa Mezhugu (VRM) (6.25 – 100 ?g/ml) for 24 hours. The results have shown that Vithu Rasa Mezhugu (VRM) induced cytotoxicity in cancer cells in a dose- dependent manner, as measured by MTT assay. It was found that the number of cells decreases as the dose increases and at approximately 109.985517?g/mL dose, 50% of the cells (HeLa cells) were less as compared to the normal control. LC50 was found to be 109.9 ?g/ml which restricted the proliferation of the HeLa cell lines after 24 hours. Apoptosis effect of VRM by AO and EtBr Double Staining results revealed that VRM has promising cell apoptotic and necrotic activity, which may help in the destruction of cancer cells in the treatment of cervical cancer. Keywords: Vithu Rasa Mezhugu, A Siddha Herbo-mineral formulation, In-vitro anti- cancer activity, HeLa cell line
Publication date: 01/08/2023

https://ijbpas.com/pdf/2023/August/MS_IJBPAS_2023_7360.pdf

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https://doi.org/10.31032/IJBPAS/2023/12.8.7360