ABSTRACT

A simple, sensitive and specific HPTLC technique for the quantitative determination of Quercetin in Psidium guajava leaves was developed. TLC Silica gel 60 F254 as stationary phase, Toluene: Ethyl acetate: Acetic acid (5:3:2v/v/v) as mobile phase, dosage speed of 16?L/sec, and detection at 254nm. The Rf value of Quercetin was found to be 0.660. According to ICH criteria, the developed technique was validated for system suitability, linearity, accuracy, precision, Limit of Detection (LOD), Limit of Quantification (LOQ), and robustness. Quercetin linearity was 2000-2800ng/spot with a correlation coefficient of 0.9994. The % recovery ranged between 98-102%, and the % RSD was <2. As a result, the developed methodology can be used for routine analysis. Keywords: Quercetin, Psidium guajava, HPTLC
Publication date: 01/08/2023

https://ijbpas.com/pdf/2023/August/MS_IJBPAS_2023_7359.pdf

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https://doi.org/10.31032/IJBPAS/2023/12.8.7359