ABSTRACT

Vitamin B12 deficiency is wide spread in all ages, races, economic classes, and both sexes population. It is arguably the most common nutritional deficiency causing injury in the United States. Early diagnosis and treatment is critical to prevent neurologic injury, disability, poor outcomes, and premature death. B12 deficiency causes demyelinating nervous system disease, dementia, psychiatric illness, anemia, vascular occlusions, fall- related trauma, suppressed immune system, and bone marrow failure. The aim of this study was to develop an in situ gel forming oral spray formulation for buccal administration of Vitamin B12. The formulation was developed using poloxamer 407 in the range of 10 % to 14 % and 1 % poloxamer 188 as themo sensitive gelling agents. Gelatin, 1 % to 2 % and 1 % HPMC E5 were used to provide mucoadhesive properties. Nine different formulation were prepared using 32 factorial design and evaluated for pH, viscosity, density, gelling strength and in vitro diffusion. The optimization of formulation revealed that the formulation F8 was showing good gelling properties at body temperature and also able to demonstrate good in vitro diffusion across semipermeable membrane. Keywords: Vitamin B12, in situ gel, oral spray, poloxamer 407, poloxamer 108. poloxamer 188
Publication date: 01/08/2023

https://ijbpas.com/pdf/2023/August/MS_IJBPAS_2023_7313.pdf

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https://doi.org/10.31032/IJBPAS/2023/12.8.7313