ABSTRACT

Citrus Medica belongs to Rutaceae family is available all over India. The main objective of the present study was to evaluate the radical scavenging potential and antiobesity activity of aqueous extract of Citrus medica peel (CMP) and its combination as peel and pulp (CMPP) . The total phenol content of the extracts reported 80.48 mg GAE/g and 84.14 mg GAE/g in CMP and CMPP with gallic acid as standard, total flavonoid content showed 49.85 mg RU/g and 56.03 mg RU/g in CMP and CMPP with rutin as standard, Vitamin C content exhibited 77.19 mg AAE/g and 83.15 mg AAE/g in CMP and CMPP with ascorbic acid as standard. The radical scavenging activity by DPPH method reported 51.77 %, 56.70%, FRAP exhibited 80.55 %, 83.33 % and NO method showed 13.19 %, 14.45 % respectively for CMP and CMPP with ascorbic acid as standard. The results of antiobesity activity by pancreatic lipase inhibitory method was found to be 9.136 ±0.845 %, 15.67±1.709 %, 26.534±2.849 %, 31.491±4.462 %, and 40.855±2.586 % for CMP extract, while CMPP extract reported 5.565±0.087 %, 28.661±0.346 %, 39.677±0.087 %, 49.535±0.435 %, and 61.709±0.248 % at concentration 0.2mg -1mg using orlistat as standard. The study showed an increase in activity with an increase in concentration of extract. On the determination of IC50 standard orlistat reported the lowest value of 0.039±0.0003 mg/ml, followed by CMPP extract of 0.795±0.004 mg/ml and CMP extract of value 1.234±0.104 mg/ml. The lower the IC50 value, the higher the efficiency of inhibiting pancreatic lipase. The study demonstrated higher antioxidant activity and antiobesity activity in CMPP than CMP. Keywords: Citrus medica, Antioxidant activity, Antiobesity activity, Pancreatic lipase
Publication date: 01/08/2023

https://ijbpas.com/pdf/2023/August/MS_IJBPAS_2023_7229.pdf

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https://doi.org/10.31032/IJBPAS/2023/12.8.7229