ABSTRACT

Extractive spectrophotometric methods that are simple, exact, sensitive, and repeatable have been devised for the detection of sitagliptin phosphate (SGP) in bulk drugs, tablets, and spiked human urine. Using a Rhodamine B solution (0.1%) and an acid phthalate buffer with a pH of 2.4, the indicated processes are based on estimation of the medication and tablet formulation. In order to analyse the spiking urine of pure drug and tablet formulation, respectively, Patent Blue vf and Methyl Orange solution are taken. After a chloroform extraction, these solutions showed absorbance maxima at 580 nm for the pure drug and tablets, 633 nm for the pure drug spiked with urine, and 427 nm for the formulations of spiked urine tablets. The analytical results for the above-described methods were statistically validated using statistics and ICH recommendations. All the factors were investigated in order to enhance the reaction circumstances. No influence was seen when common pharmaceutical excipients were present. The efficacy of the approaches was investigated by looking at the material in its therapeutic forms. Recovery rates were very good. Keywords: Ion-pair color complex, SGP, MO, Rhodamine B, Patent blue vf, methyl orange & Molar Absorptivity
Publication date: 01/07/2023

https://ijbpas.com/pdf/2023/July/MS_IJBPAS_2023_7314.pdf

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https://doi.org/10.31032/IJBPAS/2023/12.7.7314