ABSTRACT

Ulcerative colitis is equated with Pitta-Kaphaja Grahani in Ayurveda with Piccha basti as the preferred treatment. Hence the present study was undertaken for evaluating of Piccha basti in experimental colitis. Experimental colitis was induced in C57BL/6 mice with 1ml intra colonal 2% acetic acid (1 mL). Piccha basti was administered in both preventive and therapeutic model for 8 and 16 days. Colitis score, colon weight/length ratio, Disease Activity Index along with mucosal anti- oxidants-SOD, CAT, GSH, LPO, and myeloperoxidase activity, TNF?, IL-6, IL-1? and histopathological examinations were assessed. Results: Remarkable and statistically significant changes occurred in both macroscopical and cytokine parameters after acetic acid administration in positive control group in comparison to normal control in both preventive and therapeutic models except for IL6 which showed decrease in therapeutic model. The altered parameters were found to be significantly reversed by Piccha basti (Piccha basti) of both 8 & 16 days duration and standard treatment (Mesalmine enema) except that IL6 lowering was non-significant (p0.05) in Piccha basti 8 days group. Anti-oxidant parameters and MPO did not exhibit significant changes among the groups. Histopathology examination showed moderate reversal of the cytoarchitectural disturbances in PB-8 and 16 days groups in preventive model and good reversal in standard group. Reversal of the histopathological changes were found to be much better in therapeutic model. Conclusion: Based on the results it is concluded that Piccha basti for 8 and 16 days in both preventive and therapeutic models has good anti colitis effect. The effect is higher in therapeutic model and corroborated Piccha basti clinical indication of colitis like conditions. Keywords: Ulcerative colitis, Piccha basti, C57BL/6 mice, Mesalamine enema
Publication date: 01/07/2023

https://ijbpas.com/pdf/2023/July/MS_IJBPAS_2023_7296.pdf

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https://doi.org/10.31032/IJBPAS/2023/12.7.7296