ABSTRACT

Spinacia oleracea L. (child spinach) is a fairly young Amaranthaceae family leaf vegetable. The crop is high in human supplements and includes considerable quantities of bioactive mixtures such as nutrients A, C, and minerals. PGPR bacteria from Spinacia oleracea grow soil are isolated and biochemical characteristics are identified in this study. The parameter of soil was analysed. Genomic DNA was extracted from cultured bacteria. The isolated genomic DNA was amplified using the 16s rRNA region. The new DNA sequences were identified at the genus and species levels with the BLAST software tool (NCBI, USA) and deposited in (The National Center for Biotechnology Information) NCBI, with accession numbers acquired from Genbank. MEGA 11.0 (Molecular Evolutionary Genetics Analysis) was used to perform phylogenetic analysis on the DNA sequences that were most similar to the query sequences. The phylogenetic tree was generated based on the Neighbour-joining method. Keywords: Spinacia oleracea, Amaranthaceae, soil, 16s rRNA region, NCBI, MEGA 11.0.
Publication date: 01/07/2023

https://ijbpas.com/pdf/2023/July/MS_IJBPAS_2023_7208.pdf

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https://doi.org/10.31032/IJBPAS/2023/12.7.7208