ABSTRACT

Bacillus licheniformis produces lipopeptides biosurfactant that have a great potential for biopharmaceutical and biotechnological applications. Surfactin is the most important lipopeptide biosurfactant known. In this work, the lipopeptide biosurfactant produced by Indian marine bacteria, Bacillus licheniformis was analyzed. After 300 h cultivation in Zoball marine medium, a crude biosurfactant concentration of 2 g/L was produced. Biosurfactant product characterization have displayed high stability product in the wide range variation of pH and salinity. Evaluation of antimicrobial activity of biosurfactant against six human pathogenic microbes (Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Streptococcus pnemoniae, Klebsiella pneumoniae, and Salmonella typhi) demonstrated that biosurfactant produced by B. licheniformis have a specific antibacterial activity. Highest zone of inhibition was found against Staphylococcus aureus and Pseudomonas aeruginosa. Lowest zone of inhibition was found against Escherichia coli, Streptococcus pnemoniae and Salmonella typhi. Derived biosurfactant was concluded that the potential antibacterial agent against Staphylococcus aureus and Pseudomonas aeruginosa. Keywords: Bacillus licheniformis, biosurfactant, antibacterial, Staphylococcus aureus, Pseudomonas aeruginosa
Publication date: 15/06/2023

https://ijbpas.com/pdf/2023/June/MS_IJBPAS_2023_JUNE_SPCL_1082.pdf

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https://doi.org/10.31032/IJBPAS/2023/12.6.1082