ABSTRACT

Plastic and polythene waste accumulating in the environment due to their high molecular weight, hydrophobic nature, high C-C crosslink bonding, make it non-biodegradable and harmful to environment and living organisms. In the present study, 19 bacteria were isolated from dumped garbage soil on nutrient agar at 37°C, after 24h.Among nineteen, eight bacterial isolates were efficiently hydrolyzed hydrocarbons (Starch, gelatin) evaluated by zone of hydrolysis (8 to 29 mm of starch, 14 to 38mm of gelatin). Tween 80, Petroleum ether, Naphthalene powder are proficiently utilized by I8, I10 and I13 bacteria and consortium for their growth and it's confirmed their ability to LDPE biodegradation. Bacterial isolates were identified as Bacillus subtilis, Bacillus amyloliquefaciens, and Bacillus licheniformis as per Bergey's Mannual, ABIS software and 16s r RNA sequencing. Biodegradation of LDPE 81±3.2% weight loss by Bacillus licheniformis, 76.77±1.2% by Bacillus subtilis ,76.88±2.6% by consortium and lowest weight loss 16.18±2.9% was by Bacillus amyloliquefaciens in without glucose minimal media. LDPE utilization (biodegradation) for growth by bacteria was also analyzed by colorimetric method. Biodegradation of LDPE was confirmed by FTIR analysis and GC-MS. Hence in present work, LDPE sheets was biodegraded by isolates eco-friendly, helps in reducing environment pollution. Keywords: LDPE, FTIR, Biodegradation, Hydrocarbons
Publication date: 15/06/2023

https://ijbpas.com/pdf/2023/June/MS_IJBPAS_2023_JUNE_SPCL_10251.pdf

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https://doi.org/10.31032/IJBPAS/2023/12.6.1025