ABSTRACT

A simple, rapid, economical, precise and accurate Stability indicating RP-HPLC method for simultaneous estimation of Moxifloxacin Hydrochloride and Loteprednol Etabonate in Their Combined Dosage Form has been developed. A reverse phase high performance liquid chromatographic method was developed for the simultaneous estimation of Moxifloxacin Hydrochloride and Loteprednol Etabonate in Their Combined Dosage Form. The separation was achieved by LC- 20 AT C18 (250mm x 4.6 mm x 2.6 ?m) column and Buffer (Potassium Phosphate, pH 3.0): Acetonitrile (60:40) as mobile phase, at a flow rate of 1 ml/min. Detection was carried out at 229 nm. Retention time of Loteprednol Etabonate and Moxifloxacin Hydrochloride were found to be 3.550 min and 5.307 min respectively. The method has been validated for linearity, accuracy and precision. Linearity observed for Moxifloxacin Hydrochloride 5-15 ?g/ml and for Loteprednol Etabonate 5-15 ?g/ml. Developed method was found to be accurate, precise and rapid for simultaneous estimation of Moxifloxacin Hydrochloride and Loteprednol Etabonate in their Combined Dosage Form. The drug was subjected to stress condition of Hydrolysis, Oxidation, Photolysis and Thermal degradation. The proposed method was successfully applied for the simultaneous estimation of both the drugs in commercial Combined dosage form. Keywords: Moxifloxacin Hydrochloride, Loteprednol Etabonate, Stability indicating RP-HPLC Method, Validation
Publication date: 15/06/2023

https://ijbpas.com/pdf/2023/June/MS_IJBPAS_2023_JUNE_SPCL_1023.pdf

Download PDF
https://doi.org/10.31032/IJBPAS/2023/12.6.1023