ABSTRACT

To investigate the therapeutic effects of Antigonon leptopus root extract and its fractions of for ?-amylase and ?-glucosidase inhibitory activity by in-vitro assays. Roots of Antigonon leptopus were extracted with methanol and its fractions were prepared by dispersing uniformly in distilled water and subjected to solvent fractionation with ethyl acetate, butan-2- one and water. The extract and its fractions were concentrated under reduced pressure. The inhibitory effect of these extracts on these enzymes was determined by in vitro methods and some antioxidant parameters. The results revealed that the methanolic extract of roots and its fractions were inhibited ?-amylase and ?- glucosidase enzymes in a dose dependent manner. Among all these ethyl acetate fraction of Antigonon leptopus roots (EAAL) has shown the prominent ?-amylase and ?-glucosidase enzyme inhibitory activity with IC50 value 6.76±0.20 and IC50 value 7.58±0.21mg/ml ?-amylase and ?-glucosidase respectively and it was well comparable with standard acarbose (with IC50 values 5.7±0.29 and 6.95±0.80mg/ml respectively). Phytochemical reports of this plant revealed that it contains alkaloids, flavonoids, carbohydrates, tannins, saponins, steroidal and phenolic compounds. Further, the total phenolic and total flavonoid contents were also estimated. These results substantiate the use of Antigonon leptopus in traditional medicine for the treatment of diabetes by controlling postprandial hyperglycemia. The results suggest that ethyl acetate fraction (EAAL) of roots shown significant ?-amylase and ?-glucosidase enzyme inhibitory activity. High total phenolic content and total flavonoid content of root may be responsible for enzyme inhibition by regulating the glucose absorption. Keywords: Antigonon leptopus, ?-amylase, ?-glucosidase, Acarbose, quercetin, Total Phenolic Content, Total Flavonoid Content
Publication date: 01/06/2023

https://ijbpas.com/pdf/2023/June/MS_IJBPAS_2023_7187.pdf

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https://doi.org/10.31032/IJBPAS/2023/12.6.7187