ABSTRACT

Natural compounds have a long history of being used as anticancer and antioxidant agents. This work aimed to find out about the phytochemical contents, antioxidant activity, and in vitro cytotoxicity of B. variegate, L. camara, C. papaya, and V. negundo against HeLa and KB cell lines. The plant leaves were dried, powdered, and extracted by cold percolation with methanol for 24h. Total flavonoid content and total phenolic content were analyzed by phytochemical screening. All extracts assessed for antioxidant activity were examined against ABTS and the DPPH radical. Using a cytotoxicity assay, they tested the crude extracts against HeLa and KB cell lines. V. negundo had the highest TFC, measuring 58.19±2.04 mg of quercetin equivalent, and L. camara had the highest TPC, measuring 191.7±2.17 mg of gallic acid equivalent. The extracts exhibited an IC50 value of 53.13±2.24 ?g/ml of the C. papaya methanolic extract compared to the IC50 value of 19.65±0.88 ?g/ml, as shown by the Ascorbic acid. L.camara leaves have a higher superoxide scavenging effect with an IC50 value of 39.5±1.15 ?g/ml compared to the IC50 value of 13.21±2.13 ?g/ml, as shown by the BHT. The B. variegate methanolic extract exhibited effective cytotoxicity with an LD50 of 78.91 ?g/ml for HeLa cells. C. Papaya methanolic extract exhibited effective cytotoxicity with an LD50 of 97.96 ?g/ml for KB cells. With the data on the total flavonoid, phenolic, and cytotoxic activity of B. variegate and C. papaya leaves extracts, these species could be evaluated as possible sources of anticancer compounds. Keywords: Medicinal plants, Total phenolic and flavonoids, Antioxidant activity, Cytotoxic activity on HeLa and KB cell lines
Publication date: 01/06/2023

https://ijbpas.com/pdf/2023/June/MS_IJBPAS_2023_7179.pdf

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https://doi.org/10.31032/IJBPAS/2023/12.6.7179