ABSTRACT

The objective of this experimental work is the development and validation of stability- indicating the HPTLC method for the determination of Brivaracetam. A simple, fast, specific, rugged, and reproducible analytical method was developed and validated on a Camag HPTLC machine programmed with Camag Linomat 5 sample applicator Camag TLC 3 detector. The stationary phase used was precoated aluminium plates of silica gel 60F254 plates (10 cm ×10cm) with 250?m thickness, mobile phase used in the ratio of Toluene: Acetone: Methanol (6:2:2), saturated for15 min at room temperature (25°C±2) followed by densitometric scanning at 222nm. The developed protocol was most accurate, reproducible, and detectable towards Brivaracetam without any unwanted interference. When evaluated on various parameters like system suitability, precision, accuracy, linearity, specificity, robustness, LOD, and LOQ, force degradation study, the method is found to be efficient. Keywords: Method Validation, HPTLC method, Brivaracetam, System suitability.
Publication date: 01/05/2023

https://ijbpas.com/pdf/2023/May/MS_IJBPAS_2023_7123.pdf

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https://doi.org/10.31032/IJBPAS/2023/12.5.7123