BIOANALYTICAL METHODS FOR ESTIMATION OF GLICLAZIDE IN HUMAN PLASMA Authors: Damle M* And Pansare M
ABSTRACT
The objective of this study is to estimate gliclazide in human plasma by both HPLC and
HPTLC method. For HPLC method, SunQ C18 column was used as stationary phase and
Methanol: 0.1% Formic acid in Water was used as mobile phase in ratio of 70:30 v/v. For
HPTLC method, TLC plate precoated with silica gel 60 F254was used as stationary phase and
mobile phase selected was Toluene: Methanol: Ethyl acetate in ratio of 7:2:1 v/v/v. Simple
protein precipitation method was employed as sample pretreatment in both HPLC and
HPTLC methods. The detection wavelength set was 228 nm. Molnupiravir and Glipizide
were used as internal standard for HPLC and HPTLC method respectively. With optimised
HPLC method, Gliclazide eluted at 7.5±0.2 mins and the Rf value found was 0.49±0.06 for
HPTLC. Both the methods were found to be linear over the range of 2-10 ?g/ml. The r2 value
found for HPLC and HPTLC method was 0.9842 and 0.9835 respectively. Both of the
methods were validated for as per USFDA guidelines for bioanalytical method. The
developed methods were found to be simple, accurate, precise and economical for estimation
of Gliclazide in human plasma.
Keywords: Gliclazide, HPLC, HPTLC, Internal Standard, Bioanalytical Publication date: 01/04/2023 https://ijbpas.com/pdf/2023/April/MS_IJBPAS_2023_7078.pdfDownload PDFhttps://doi.org/10.31032/IJBPAS/2023/12.4.7078
